Berkeley LBL/JoyceNotebook
From 2007.igem.org
Contruction of pET3a Derivatives Containing bchD and bchI
1. Amplify Rhodobacter's gene and introduce restriction sites, which allow cloning PCR fragments into pET3a
bchD | bchI | |
---|---|---|
Restriction sites introduced when amplified by PCR | NdeI, BamHI | NdeI, BglI |