Berkeley LBL/JoyceNotebook

From 2007.igem.org

< Berkeley LBL
Revision as of 19:02, 25 October 2007 by Joyxi (Talk | contribs)

Contruction of pET3a Derivatives Containing bchD and bchI

1. Amplify Rhodobacter's gene and introduce restriction sites, which allow cloning PCR fragments into pET3a

bchD bchI
Restriction sites introduced when amplified by PCR NdeI, BamHI NdeI, BglI