From 2007.igem.org

Contruction of pET3a Derivatives Containing bchD and bchI

1. Amplify Rhodobacter's gene and introduce restriction sites by PCR (Using Phusion Polymerase), which allow cloning PCR fragments into pET3a

	bchD	bchI
length of gene fragment	1695 b.p.	1005 b.p.
Restriction sites introduced when amplified by PCR	NdeI, BamHI	NdeI, BglI