PennState/Parts
From 2007.igem.org
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New Parts (submitted) |
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xylR XylR is a protein that belongs to the AraC/XylS family. This regulator participates in controlling several genes involved in xylose utilization. XylR activates the xylAB and xylFGHR operons. |
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Promoter xylF The xylose F promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included as well as a CRP activator binding site. |
New Parts (underconstructed/unverified) |
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natural RBS xylR TT 'xylR total' XylR is a protein that belongs to the AraC/XylS family. This regulator participates in controlling several genes involved in xylose utilization. XylR activates the xylAB and xylFGHR operons. |
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CRP* CRP* is a modified CRP to not require cAMP. Crp is a dual transcriptional regulator that belongs to the CRP family. It is the major global regulator of catabolite-sensitive operons and it controls its own synthesis. This regulator induces a severe bend in the DNA and is capable of regulating the expression of more than 200 genes. |
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Promoter xylA The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included as well as a CRP activator binding site. |
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Promoter xylA without CRP site The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included, but the promoter region was cloned without the CRP binding site. Note: the removal of CRP may keep the promoter from working unless. |
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Promoter xylF without CRP site The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included, but the promoter region was cloned without the CRP binding site. Note: the removal of CRP may keep the promoter from working unless. |
