USTC/ZhaoYun
From 2007.igem.org
Contact:
Address:
Room 438, Life Science Building, University of Science and Technology of China
Hefei, Anhui, P.R.China (230027)
Email:
zhaoyun@mail.ustc.edu.cn (preference)
Tel:
+86-551-3602469
Mobile:
+86-13866776861
Research Work
My major work in iGEM is to design and biologically implement three logic promoters: NAND,NOR and NOT. I attempt to systematically build up a procaryotic promoter family whose members contain different operons, that is, operons with different nucleotide sequences and different relative locations. We tested the expression activity under various combined signals of upstream repressors, and systematically study on how different operons influence the expression activity of repressors.
In detail, I have worked on four parts. The first one is that I designed to use PCR method to build up the procaryotic promoter family. The second one is that I measured and compared different repression efficiency according to the different locations of the two operons. The third is that I measured and compared different repression efficiency according to the different nucleotide sequence of the two operons, and have found a way to alter the combination intensity of repressors. And the fourth one is that I discussed ranges of several parameters that are suitable for NAND, NOR, NOT Gate, with the help of data of DNA looping reported on previous papers. And I have attempted to synthesize and test the targeted artificial logic promoters.
