Berkeley LBL/MimiSequence
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< Berkeley LBL
Revision as of 06:59, 26 October 2007 by KonniamChan (Talk | contribs)
Sequences and Properties of Oligonucleotides:
S-chlH:
S-chlH3-kpnI-BamHI 5' - TAA AGG ATC CAA AGG TAC CTT ATT CAA CCC CTT CAA TGC GGT CTT CC - 3' TM (50mM NaCL): 66.7 °C GC Content: 44.7%
S-chlH5-ndeI 5' - AAT TCA TAT GAC TGC CAC CCT TGC CGC CCC CAG C - 3' TM (50mM NaCL): 70 °C GC Content: 58.8%
S-chlI:
S-chlI3-speI-NotI-bglII 5' - TAT TAG ATC TTT AGC GGC CGC TTT ACT AGT TTA AGC TTC ATC GAC AAC GCC AAA AAC CCG - 3' TM (50mM NaCL): 68.4 °C GC Content: 43.3%
S-chlI5-kpnI-rbs 5' - TTT AGG TAC CAA GAA GGA GAT ATA CTT ATG ACT GCC ACC CTT GCC GCC CCC AGC - 3' Properties TM (50mM NaCL): 70.5 °C GC Content: 51.9%
S-chlD:
S-chlD3-NotI-bglII-long 5' - TTG AAG CCT AGG AGA AGA GTA GGG AAT TTT TTG ACC AAT TTC TAG - 3' TM (50mM NaCL): 63.1 °C GC Content: 37.8%
S-chlD5-speI-rbs-long 5' - ATT GGG AGT CAA CTT CTA CTA AAT CTT CAG AAA AAC GAT ATT TGG - 3' TM (50mM NaCL): 61.6 °C GC Content: 33.3%