Valencia/Comparator
From 2007.igem.org
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During the brainstroming, an idea came up: mimetize an electronic comparator on a biologcal device. It still works with repressors, so it may sound familiar to most of us.
What:
Two inputs enter the system, a genetic circuit compares both signals and amplifies the higher one.
How:
So easy, I would be quite surprised if we don't find any drawback.
Two different promoters that act as sensors of different inputs and two repressors that inhibit each other.
- We have two promoters that 'sense' something: psensor1 and psensor2
- We have two promoters repressed: pLacI and ptet
- We also have two CDS of the repressors: lacI and tetR
- And, finally, we have the outputs: CFP and YFP
The system would be:
So, if we have equal amount of whatever 1 and 2, there will be equal amounts of both repressors and, So, equal amounts of CFP and YFP. If some input 1 is sensibly higher than the 2, we will have more tetR that will inhibit ptet and we will have less YFP
What for:
We would have a device that would clear the noise of a system. It can be used in other projects, as it implies only two genes and 4 promoters...
Problems:
- Will a gene driven by two different promoters work well enough?? ETH tried it with some results, why not give it a try?
- It's a small project, but it can be coupled to some other project...
- We still have to find some new BioBricks to build, as almost every single construction would be on the iGEM plate
Developed by: Arnau, Pablo and Javi