From 2007.igem.org

Contents 1 Abstract 2 Contact List 2.1 Faculty Advisors 2.2 Graduate Advisors: 2.3 Undergraduate Students 3 Useful References 4 Safety 4.1 upcoming training 4.2 log your safety training 4.3 Bindley Safety 5 Project Description and Details 6 Photo Area

Abstract

This year's Purdue 2007 iGEM team will be presenting a project called "Bacteria Warfare" for this year's competition hosted by MIT in November. The team is composed of a group of undergraduates from different majors including Biomedical Engineering, Agricultural and Biological Engineering, Biochemistry, and Chemistry. Escherichia coli (E. coli) is used to conduct the microbial warfare. The design is to use two different types of transformed E. coli. One type of E. coli produces protein that triggers the death gene of its opponent (but not for itself) which expresses the production of a protein toxin. One expresses green fluorescence protein and the other expresses red fluorescence protein so that the progression of the war between types can be easily viewed and monitered. The team decided the general topic of a bacteria wafare at the end of spring 07 and began to design and construct the wafare in summer 07.

Contact List

Faculty Advisors

• Dr. Rickus rickus@purdue.edu
  
• Dr. Clase
  
• Dr. Mosier
  

Graduate Advisors:

• John Schumm jschumm@purdue.edu
  
• Alex Dimauro adimauro@purdue.edu

Undergraduate Students

• Aaron Young ajyoung@purdue.edu
  
• Meng Zhou zhoum@purdue.edu
  
• Janie Stine mstine@purdue.edu
  
• Dan Sheik dsheik@purdue.edu
  
• Craig Barcus

Useful References

[http://intl-mmbr.asm.org/cgi/reprint/64/3/503 Programmed Cell Death in Bacteria]

[http://www.springerlink.com/content/n88p472061740k32/fulltext.pdf Spiteful Bacteria ... Who wins?]

[http://jb.asm.org/cgi/reprint/94/4/1093 colicin tolerant E coli mutants]

[http://www.nature.com/nature/journal/v428/n6981/pdf/nature02429.pdf Bacterial Rock Paper Scissors]

Safety

upcoming training

If you have not completed safety training in the last 12 months then please attend the BMED safety training on May 24, 1:30 - 3:30 PM Thursday in BMED 2001.

log your safety training

Work in Lab and should have already compeleted safety training:

• Meng Zhou
• Dan Sheik

Bindley Safety

Finally, for safety information specific to the labs in Bindley see the [http://openwetware.org/wiki/Rickus_Lab_Safety Rickus Lab Safety] page on openwetware

Project Description and Details

We transformed two types of E. Coli: one with plasmid 1 and the other plasmid 2.

the gene map can be find from IGEM parts: BBa_I738009 (for plasmid 1) and BBa_I738010 (for plasmid 2)

Photo Area

Our home building on Campus :

[http://www.cyto.purdue.edu/flowcyt/staffpgs/bindly%20for%20ppt.jpg Bindley Bioscience Center]