Project
This project aims to use light to form a solid fluorescent mass of E. coli where two light beams intersect in a suspension of cells. We've called our building system "Coliforming".
Motivation
Possible applications in building complex scaffolds in tissue engineering.
Requirements
The system requires six biological components.
Red photosensor
Blue photosensor
AND gate
GFP fluorescent reporter
Promotable surface expression of cadherins
Gas vesicle expression to produce neutrally bouyant bacteria
High Level System Design
Induce or constitutively express gas vesicles for neutral bouyancy
If Red & Blue light detected, then promote adhesion molecules.
