Caltech/Protocols/BioAssay Buffer

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iGEM 2007

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To 1L ddH20 you will add:

  • 0.5 g Tryptone
  • 0.3 mL 100% Glycerol (or, I use 0.6 mL sterile 50% glycerol)
  • 5.8 g NaCl
  • 5.0 mL 1M MgSO4

Autoclave to sterilize. Small aliquots are preferred, since contamination is difficult to detect.

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