Waterloo

Drawing on our diverse backgrounds, we bring a wide range of skills and modes of creative thinking to our iGEM project. The iGEM competition is providing us with an opportunity to become more familiar with the emerging field of synthetic biology in an engaging and fun atmosphere. In addition to gaining experience in the design, construction, and analysis of genetic circuits, we are also meeting the challenge of bringing together a large, diverse group toward a common goal.

- goals - biological implementation of a fundamental engineering concept, the half-adder - half adder calculates the sum of two 1-bit inputs project - bacterial sensor which responds to two inputs: red light and the chemical tetracycline - input sensors control genetic switches in order to carry out logic functions - output of the half-adder function...green or red fluorescence - applications - why is it important?

• Binary addition and boolean logic
• Half-adder vs. full-adder designs
• Biological half-adder implementation

//Modelling

• Strategy for half-adder construction
• Testing plan and testing constructs

• Plan for full-adder
• Gene design for full adder

• Dr. Trevor Charles
• Dr. Barbara Moffatt
• Dr. Joshua Neufeld