From 2007.igem.org

Experiments

Microscopy:

• pT luxI Cfp imaging done at various induction levels but OD was too high
• K12Z1 imaging done at various induction levels
• 1st Open loop trial Experiment DONE (Auto fluorescence, pT.luxI.C (at AI OD 2 and 0.2))

FACS:

• Auto fluorescence at inductions [0,1,10,25,50,100] done in triplets
• pT.luxI.C fluorescence at inductions [0,1,10,25,50,100] done in triplets
• Fluorescence due to M9 solution (Control)

Analysis

Microscopy:

pT LuxI.C: Subtract K12Z1 and plot CFP,YFP,RFP vs aTC concn

Red : K12Z1 Fluorescence values at different aTc induction levels

Green : pT.luxI.C Fluorescence values at different aTc induction levels

Blue : pT.luxI.C - K12Z1 Fluorescence values at different aTc induction levels

Linear and log space

gallery