June 20
From 2007.igem.org
Experiments
Microscopy:
- pT luxI Cfp imaging done at various induction levels but OD was too high
- K12Z1 imaging done at various induction levels
- 1st Open loop trial Experiment DONE (Auto fluorescence, pT.luxI.C (at AI OD 2 and 0.2))
FACS:
- Auto fluorescence at inductions [0,1,10,25,50,100] done in triplets
- pT.luxI.C fluorescence at inductions [0,1,10,25,50,100] done in triplets
- Fluorescence due to M9 solution (Control)
Analysis
Microscopy:
pT LuxI.C: Subtract K12Z1 and plot CFP,YFP,RFP vs aTC concn
Red : K12Z1 Fluorescence values at different aTc induction levels
Green : pT.luxI.C Fluorescence values at different aTc induction levels
Blue : pT.luxI.C - K12Z1 Fluorescence values at different aTc induction levels
Linear and log space
Mean and Standard Error Plots at all induction levels
 A linear plot of mean CFP Fluorescence values at |
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