Berkeley LBL/Methods
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Experimental
Constructs
The T7 expression vector pET3a was used for the subcloning of the genes for magnesium-chelatase. The following constructs were built by inserting varioaus fragments into pET3a.
Rhodobacter sphaeroides Synechocystis sp. Heliobacillus mobilis
Protocols
PCR (Using Phusion Polymerase)
PCR (Using TaKaRa Ex Taq Polymerase)
Digestion for PCR Product or Miniprepped DNA
KCM Competent Cell Transformation
Electroporation Transformation
