PennState/Parts
From 2007.igem.org
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xylR XylR is a protein that belongs to the AraC/XylS family. This regulator participates in controlling several genes involved in xylose utilization. XylR activates the xylAB and xylFGHR operons.[BioCyc] |
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Promoter xylF The xylose F promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included as well as a CRP activator binding site.[BioCyc] |
New Parts (underconstructed/unverified) |
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natural RBS xylR TT 'xylR total' XylR is a protein that belongs to the AraC/XylS family. This regulator participates in controlling several genes involved in xylose utilization. XylR activates the xylAB and xylFGHR operons.[BioCyc] |
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CRP* CRP* is a modified CRP which is cAMP independent. CRP is a dual transcriptional regulator that induces a severe bend in the DNA and is capable of regulating the expression of more than 200 genes. It is the major global regulator of catabolite-sensitive operons and it controls its own synthesis.[Hanamura92] |
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Promoter xylA The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included as well as a CRP activator binding site.[Song97] |
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Promoter xylA without CRP site The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included, but the promoter region was cloned without the CRP binding site. Note: the removal of CRP may keep the promoter from working unless the promoter is strengthened.[Song97] |
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Promoter xylF without CRP site The xylose F promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included, but the promoter region was cloned without the CRP binding site. We believe the xylF promoter is stronger than the xylA promoter. Note: the removal of CRP may keep the promoter from working unless the promoter is strengthened.[Song97] |
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Two way promoter xylA-F with CRP site The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included and a CRP binding site. [Song97][Laikova01] |
References
*Biocyc: Nucleic Acids Research 33:D334-7 2005Nucleic Acids Research 33:D334-7 2005*Hanamura92: Hanamura A, Aiba H (1992). "A new aspect of transcriptional control of the Escherichia coli crp gene: positive autoregulation." Mol Microbiol 1992;6(17);2489-97. PMID: 1328816
*Song97: Song S, Park C (1997). "Organization and regulation of the D-xylose operons in Escherichia coli K-12: XylR acts as a transcriptional activator." J Bacteriol 1997;179(22);7025-32. PMID: 9371449
*Laikova01: Laikova ON, Mironov AA, Gelfand MS (2001). "Computational analysis of the transcriptional regulation of pentose utilization systems in the gamma subdivision of Proteobacteria." FEMS Microbiol Lett 205(2);315-22. PMID: 11750821
