From 2007.igem.org

Contents 1 Experimentation 1.1 Protocols 1.2 Assembly Line Flow 1.3 Bio-Brick Creation

Experimentation

Protocols

For reliability, a majority of our actual experimentation has followed protocols provided by the supplier of relevant projects, including enzymes ([http://www.neb.com/ NEB]) and kits ([http://www.qiagen.com/ Qiagen]). The [http://www.qiagen.com/ Qiagen] maxiprep kit was replaced with a [http://www.bio-rad.com/ Bio-Rad] kit to obtain higher yields with lower processing times.

Please consult the relevant vendors for protocol documents.

Assembly Line Flow

Bio-Brick Creation

Design Primers

PCR

Run Gel

- Check Primer concentration
- Check Primer design

Gel Extraction and determine concentrations

Digestion with Restriction Enzymes

PCR purification and determine concentrations

Ligation