From 2007.igem.org

VParker 16:37, 22 June 2007 (EDT) Big day planned. I have to PCR my tubes V1-3, V2-4, V5-7, and V6-8. these are the 4 parts to my two genes. V1-3 is Part A of my otsA gene, it ends at the internal restriction BamHI site.

Vparker 16:02, 25 June 2007 (EDT) Today i came into the lab and found that 3 out of the 4 PCR tubes were in the freezer but that i have lost the tube marked V6-8. i had the PCR products from the tubes V1-3, V2-4, and V5-7. After having fumed a bit over my lost PCR i went to work on setting up a new PCR tube for V6-8 and to also clean tubes V1-3, V2-4 and V5-7. Austin was kind enough to share the PCR machine with me and i put in the tube V6-8 in and also the sewing reaction i was working on named V1-4. V1-4 is a combination between V1-3 and V2-4. Since V1-3 and V2-4 are two parts of one gene and i had to PCR out the internal restriction site. File:New Image.jpg