From 2007.igem.org

This year our new team has been developing the project established last year which you can see on the McGill 2006 wiki. We have made many new discoveries and have run into some problems hindering our progress along the way; nevertheless, we were able to make some new developments as outlined below:

Reproducing Last Year's System

The primary oscillating system, the I15004 with the J40001 as outlined in the previous pages was produced last year and viewed using a microscope for several hours to analyze the oscillatory period and confirm the presence of these oscillations. Click here for methods

From these experiments, we were able to come up with some interesting results.

1. The more concentrated the cells, the worse the apparent oscillations.
2. The presence of Dox seemed to stabilize the oscillations.
3. The greater the amount of AHL, the worse the apparent oscillations, as predicted with the simulations.
4. Using an optical density assay, it was found that the cells were in fact dying (or clumping) thus lowering the optical density throughout the testing period.
5. Oscillations occured with the J40001 (pLux-LacI-ECFP) alone. The reason for this could be some interaction with the expression of endogenous Lac in the BL21 cell line we used.

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Production of a new I15004 biobrick