Project 1 Mexican Wave

FIRST VIEW

Our first thought was to create a pattern by the change of colors in a concentration dependent manner; so that the production of a protein at the end of a different event would trigger the pattern formation, just like a goal triggering a *Mexican wave in soccer games.
The plan was to have a plate having a lawn of same type E. coli cells having 3 constructs. Each construct would have a promoter activated by a different level of the initial signal protein and a reporter fluorescent protein coding part.
The triggering protein A is being dropped on the middle of the plate, creating a concentration gradient on the plate as in the figure. Acording to this plan the color change will be once, and then by the rising level of [A], the plate will all become green.

SECOND VIEW

The second view was to make this color change continuous and independent of a created concentration gradient. So first idea came up to be coupling this oscillation to an already oscillating protein, maybe to cell cycle. But then this oscillation will be observable only on a single cell unless we synchronize the cell cycles of different cells on the plate.

Mexican Wave