From 2007.igem.org

Results

Invitrogen BenchMark Protein Ladder

Plasmid Key for Protein Gels

Discussion

Prior to running the protein gels, we expected to see protein bands for the constructs that contained the -H and -I gene(~140kDa and ~38kDa, respectively) and were also induced with IPTG . We did not expect to see bands that coded for the -D gene (~70kDa) and for all constructs that were not induced with IPTG. Although the induced constructs that contained the -H gene had strong bands at ~140kDa, the uninduced constructs also showed strong bands in the same area. This gene was able to be expressed with or without induction of IPTG. In addition, protein bands that coded for the -I gene showed a strong band; however, when expressed with the -H gene, it did not clearly show a strong band at ~38 kDa in comparison to the benchmark ladder. As expected, protein bands for the -D gene also did not show, alone nor in conjunction with the -H and -I gene. Although our protein gels did not convey expression of neither the -I nor -D genes, our genes may still be expressed and may have enough activity to catalyze the Mg-chelatase enzyme to produce Mg-protoporphyrin IX.