From 2007.igem.org

Project 1 Mexican Wave

FIRST VIEW

• Our first thought was to create a pattern by the change of colors (by production of different fluorescent proteins) triggered by a signalling protein in a concentration dependent manner; so that the production of a protein as a result of the processing of a separete system, would trigger the pattern formation, just like a goal triggering a *Mexican wave in soccer games.

• The idea was to have a plate having a lawn of cloned E. coli cells having 3 constructs. Each construct would have a promoter, which would be activated by a different level of the initial signal protein, and a coding part for a reporter fluorescent protein.

• The triggering protein (say protein A) would be dropped at the middle of the plate, creating a concentration gradient on the plate as it diffuses. According to this, the color change will be for once, and then by the rising level of [A], the plate will all become green.

SECOND VIEW

• The second view was to make this color change continuous and independent of a concentration gradient gradient created by us. So first idea came up to be coupling this oscillation to an already oscillating protein in the cell, such as a cell cycle protein. But then this oscillation will be observable only on a single cell unless we synchronize the cell cycles of different cells on the plate.

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