NYMU Taipei/Construct
From 2007.igem.org
Assembly Ordering and Progress
system 1
System 1: glucose-sensing insulin generator (5 task left: 2 PCR and 3 biobrick standard assembly) |
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pOmpC | RBS + TAT |
INSA | RBS + TAT |
INSB | RBS | CinR | RBS | HSL | D-term | duration | members | assembly | status |
RBS + CinR | RBS + HSL | 8/30-8/31 | 翔 | 1st assembly | 9/01, 07 |
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CinR + HSL (RBS+) | 9/1-9/4 | 宥,君,威 | 2nd assembly | 9/02, 07 |
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CinR+HSL(RBS+) + D-term | 9/16-9/27 | 宥,君,翔,pett | 3rd assembly | 9/27 07 |
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RBS + TATA+ INSA (PCR) | RBS + TATB+ INSB (PCR) | 9/28-9/30 |
銘, pett |
basic part (PCR) | 9/30, 07 |
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pOmpC + TATA_INSA (failed) | TATB_INSB + CinR+HSL+D-term (wrong assembly) |
-10/5 | pett | 4th assembly | 10/5,7,8 07 |
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pOmpC + TATA_INSA | -10/11 | pett | 4th assembly | ||||||||||
pOmpC + TATA_INS_A + TATB_INS_B |
-10/23 | 4th assembly | |||||||||||
pOmpC + TATA_INS_A + TATB_INS_B + CinR+HSL+D-term | Final |
system 2
System 2: insulin-sensing insulin destoryer (5 tasks left: 2 PCR and 3 biobrick standard assembly) |
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pCinRHSL | OmpRBS | RBS + TATD |
IDE | D-term | duration | members | assembly | status |
OmpRBS (PCR) | RBS + TATD+ IDE (PCR) |
-102 |
銘, 茹, pett |
basic part (PCR) | 10/2 07 |
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pCinRHSL + OmpRBS | -10/5 | pett | 1st assembly | 10/5 07 |
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pCinRHSL + OmpRBS + TATD_IDE (RBS+) | -10/11 | pett | 2nd assembly | |||||
pCinRHSL+OmpRBS+TATD_IDE + D-term | Final |
Promoter assay construct: pOmpC+(RBS+EYFP)
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OmpRBS assay construct: pLacIQ + OmpRBS + GFP
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OmpRBS assay construct: pLacIQ + OmpRBS
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System 1: pOmpC + (TATA+INSA) + (TATB+INSB) + (RBS+CinR) + (RBS+HSL) + D-term
Phase 1 of system 1: RBS + CinR
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Phase 1 of system 1: RBS + HSL
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Phase 1 of system 1: TATA + INSA = TATA_INSA
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Phase 1 of system 1: TATB + INSB = TATB_INSB
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Phase 2 of system 1: pOmpC + TATA_INSA
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Phase 2 of system 1: (RBS+CinR) + (RBS+HSL) = CinR+HSL
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Phase 3 of system 1: pOmpC + TATA_INSA + TATB_INSB
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Phase 3 of system 1: CinR+HSL + D-term = CinR+HSL+D-term
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Phase 4 of system 1: pOmpC + TATA_INSA + TATB_INSB + CinR+HSL+D-term
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System 2: pCinRHSL+ OmpRBS + (TATD+IDE) + D-term
Phase 1 of system 2: TATD + IDE = TATD_IDE
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Phase 1 of system 2: pCinRHSL + OmpRBS
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Phase 2 of system 2: pCinRHSL + OmpRBS + TATD_IDE
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Phase 3 of system 2: pCinRHSL + OmpRBS + TATD_IDE + D-term
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Basic Constructs
pOmpC in pSB1A2
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pCinRHSL in pSB1A2
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RBS in pSB1A2
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D-term in pSB1AK3
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