ThpD
From 2007.igem.org
PCR ThpD F/R on ThpD (916bp, BglII/XhoI)
Sub into pBca9145-Bca1144 (BglII/XhoI, 2054+910 L)
Product is pBca9145-BBa I716334
ThpDF Forwar BglII site anneals to ThpD cggatAGATCTatgaccaccgaagtacgcgc
ThpDR Reverse XhoI site anneals to ThpD gtcagctcgagctacttcaccggggtgaagt