Wisconsin/Protocol:Ligation

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Ligation

Use Nanodrop to find vector and insert concentration in [ng/uL]. Find the length of vector and insert and calculate volume of reagents needed with [http://mywebspace.wisc.edu/klapoetke/web/iGEM/WIDGET_Ligation_Reaction.xls this excel spreadsheet].

Heat inactivate ligation afterwards: 15 minutes at 65C.

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