Melbourne/cannon plasmid pNL26 res map
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Restriction map of core enzymes from: http://arbl.cvmbs.colostate.edu/molkit/mapper/index.html
Enzyme | Cuts | --------- Base 5' to Cleave Sites ---------- |
---|---|---|
BamHI | 2 | 2916 , 5033 |
BglII | 1 | 8438 |
ClaI | 5 | 7304 , 7340 , 7698 , 9196 , 10004 |
EcoRI | 2 | 5789 , 10026 |
EcoRV | 2 | 9273 , 9491 |
HincII | 3 | 3652 , 4927 , 9757 |
HindIII | 3 | 3469 , 4121 , 8973 |
KpnI | 2 | 25 , 3408 |
NcoI | 2 | 5296 , 5365 |
NotI | 1 | 2897 |
PstI | 7 | 2937 , 5453 , 5831 , 5936 , 9073 , 9106 , 9326 |
SacI | 2 | 2884 , 7910 |
SalI | 0 | |
SmaI | 1 | 2924 |
XbaI | 1 | 2904 |
XhoI | 2 | 6 , 5345 |