From 2007.igem.org

https://static.igem.org/mediawiki/2007/f/f5/800px-Edinburgh_City_15_mod.JPG

Bacterial counter

The idea is to use recombination to cut out or flip sections of DNA and use this to count or trigger the expression of genes.

Project Goal

The aim is to produce output as a function of cell division. This can have the added potential of performing programmed cell death after a determined number of divisions. We also hope to further analyse cell division and recombinase mechanisms.

Current Device Idea

This device should output a PoPS signal every time a cell division occurs This can then be hooked up to another device such as a counter.

This device realise on div sites flipping only once per division. To test whether this actually happens or not we will build this smaller, simpler device to prove flipping occurs upon cell division and a second test to show whether it flips once or many times per division