Alberta/Calender/July

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July

July 2007
Su M Tu W Th F Sa
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31


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July 1

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July 2

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July 3

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July 4

Lab Notes:

Transformations of: J61003 (vector containing GFP), J23119 (constitutive promotor), E1010 (RFP), J45100 (methyl salicylate), 0034 (RBS). Transform into XL10 gold cells. Plate on Amp.

-ED, JG, ML, MC, VH, AL, CZ

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July 5

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July 6

Lab Notes:

Transformation of E1010 did not work, it is in a Kan vector. SMRT.

Set up 4X5mL overnights of pSB1A3, J45100, B0034, J61003.

-ED

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July 7

Lab Notes:

Glycerol stocks of overnights from July 6. Stored in -80 freezer. Miniprep from overnights started July 6. Stored in "iGEM" freezer box in -20 freezer.

-JP,JB,AL

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July 8

Lab Notes:

Transform E1010 and A0500 into XL10 Gold. Plate on Kan.

Pour Kan and Amp plates. Make TBE and TAE buffer.

-ED, JP, MC, VH, CZ

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July 9

Lab Notes:

Retransform E1010 and A0050 into XL10Gold cells using new plates that were poured Sunday.

-ED, VH, JG

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July 10

Lab Notes:

Transformations of E1010 and A0050 were not successful. Tomorrow we will try with a new cell line and enriched media.

-ED, MC, AF, CZ

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July 11

Lab Notes:

Third attempt at transforming the Kan resistant BioBricks. Transformed E1010, A0500, and pSB2K3 into XL10 Gold, XL10 Gold with enriched media (Magnesium & Glucose), and DH5a cells. Plated 100uL and the pellet remaining after taking the 100uL aliquot on Kan plates.

Restriction digest of J61003 with Xba and Spe. Run on 0.8% agarose gel. Cut out 2290 band, will gel extract tomorrow.

-ED, MC, NG, JG, VH, CZ

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July 12

General Notes:

Calender up and running.

Meeting tonight at 7:00 in CSC 2-49.

Received Chlorobium tepidum, meeting with Dr. Jeff Fuller (with Capital Health) to discuss the use of their anaerobic chambers!! -Justin

Lab Notes:

Transformations with DH5a cells worked, but we will need to select for single colonies tonight.

Streak for single colonies of E1010, A0500, J61003.

Purification of gel slice of digested J61003.

-ED, JG, AL, JB, MC, CZ

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July 13

General Notes:

Everyone who is coming tonight please be present 7:00pm sharp

Lab Notes:

Started 4X5mL overnights of E1010 and I0050 at 8:30 am. Should be ready for miniprep tonight.

-ED, JB

No growth - made some Kan plates

-MC, JP, JG, CZ

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July 14

Schedule:

AL,JP,NK

General Notes:

If you are scheduled during the day please be in the lab at 1230hrs (PM) Don't forget that the Bio-Sci doors lock at 7:00 so if you are trying to get in after 1900hrs call 492-2911

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July 15

Schedule:

Jp,JB,AL


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July 16

Schedule:

ED,VH,AF



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July 17

Schedule:

CY,JG,AF


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July 18

Schedule:

CY,MC,NG

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July 19

Meeting at 7:00, Room TBA. Schedule:

ED,MC,VH

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July 20

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July 21

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July 22

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July 23

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July 24

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July 25

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July 26

Meeting at 7:00, Room TBA.


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July 27

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July 28

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July 29

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July 30

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July 31

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