Alberta/Calender/July
From 2007.igem.org
Schedule Legend: JG: Jason, Mc: Michelle, ED: Erin, NG: Nick G., NK: Nik K., CY: Celine, AF: Adam, Al: Alex, JB: Jori, JP: Justin, VH: Veronica,
July
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July 1
July 2
July 3
July 4
Lab Notes:
Transformations of: J61003 (vector containing GFP), J23119 (constitutive promotor), E1010 (RFP), J45100 (methyl salicylate), 0034 (RBS). Transform into XL10 gold cells. Plate on Amp.
-ED, JG, ML, MC, VH, AL, CZ
July 5
July 6
Lab Notes:
Transformation of E1010 did not work, it is in a Kan vector. SMRT.
Set up 4X5mL overnights of pSB1A3, J45100, B0034, J61003.
-ED
July 7
Lab Notes:
Glycerol stocks of overnights from July 6. Stored in -80 freezer. Miniprep from overnights started July 6. Stored in "iGEM" freezer box in -20 freezer.
-JP,JB,AL
July 8
Lab Notes:
Transform E1010 and A0500 into XL10 Gold. Plate on Kan.
Pour Kan and Amp plates. Make TBE and TAE buffer.
-ED, JP, MC, VH, CZ
July 9
Lab Notes:
Retransform E1010 and A0050 into XL10Gold cells using new plates that were poured Sunday.
-ED, VH, JG
July 10
Lab Notes:
Transformations of E1010 and A0050 were not successful. Tomorrow we will try with a new cell line and enriched media.
-ED, MC, AF, CZ
July 11
Lab Notes:
Third attempt at transforming the Kan resistant BioBricks. Transformed E1010, A0500, and pSB2K3 into XL10 Gold, XL10 Gold with enriched media (Magnesium & Glucose), and DH5a cells. Plated 100uL and the pellet remaining after taking the 100uL aliquot on Kan plates.
Restriction digest of J61003 with Xba and Spe. Run on 0.8% agarose gel. Cut out 2290 band, will gel extract tomorrow.
-ED, MC, NG, JG, VH, CZ
July 12
General Notes:
Calender up and running.
Meeting tonight at 7:00 in CSC 2-49.
Received Chlorobium tepidum, meeting with Dr. Jeff Fuller (with Capital Health) to discuss the use of their anaerobic chambers!! -Justin
Lab Notes:
Transformations with DH5a cells worked, but we will need to select for single colonies tonight.
Streak for single colonies of E1010, A0500, J61003.
Purification of gel slice of digested J61003.
-ED, JG, AL, JB, MC, CZ
July 13
General Notes:
Everyone who is coming tonight please be present 7:00pm sharp
Lab Notes:
Started 4X5mL overnights of E1010 and I0050 at 8:30 am. Should be ready for miniprep tonight.
-ED, JB
No growth - made some Kan plates
-MC, JP, JG, CZ
July 14
Schedule:
AL,JP,NK
General Notes:
If you are scheduled during the day please be in the lab at 1230hrs (PM) Don't forget that the Bio-Sci doors lock at 7:00 so if you are trying to get in after 1900hrs call 492-2911
Things to be continued today (CZ) 1. Put LB plates in 4 degree fridge 2. Plate 20 microlitre of DH5 alpha on a kan plate as a control 3. SpeI (BcuI)/EcoRI double digest NOTE: Sequential digestion is needed. Start with 1 microlitre of BcuI and 2 microlitre of 10x Tango and incubate for an hour. Then add 1 microlitre of EcoRI and 2.5 microlitre of 10X Tango and incubate for an hour. 4. Miniprep on the colonies picked on July 13 at 10pm. They are in the shaker in the back room. Note the colonies picked were also streaked on kan plates in the 37 degree incubator. Erin's colonies are in the shaker in the front room.
If you need anything else call me(Celine). Number on the blackboard. Thank you.
July 15
Schedule:
Jp,JB,AL
July 16
Schedule:
ED,VH,AF
July 17
Schedule:
CY,JG,AF
July 18
Schedule:
CY,MC,NG
July 19
Meeting at 7:00, Room TBA.
Schedule:
ED,MC,VH
July 20
Schedule:
JG,ED,MC
July 21
Schedule:
JP,NK,NG
July 22
Schedule:
JP,AL,NK
July 23
July 24
July 25
July 26
Meeting at 7:00, Room TBA.
July 27
July 28
July 29
July 30
July 31
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