The image immediately to the right of this text shows the results from our transformation of E.coli with plasmids pBAD18s, pBAD30, and pjQ200 via Zymo transformation and electroporation.
The contents of the plates are as follows(column indicates transformation type):
Zymo(poured)
Zymo(streaked)
Electroporation
pBAD18s
pBAD18s
pBAD18s
pBAD30
pBAD30
pBAD30
pjQ200
pjQ200
pjQ200
The "poured" and "streaked" designations refer to how the transformed E. coli were put onto the plate. When transformed E. coli were streaked onto the plate, 5 microliters were used. When transformed E. coli were poured onto the plate, 100 microliters were used.
Shewy Transformants
The image immediately to the right of this text shows the results from our transformation of S. oneidensis with plasmids pBAD18s, pBAD30, and pjQ200 via Zymo transformation and electroporation.
The contents of the plates are as follows(column indicates transformation type):
Zymo(streaked)
Zymo(poured)
Electroporation
pBAD18s
pBAD18s
pBAD18s
pBAD30
pBAD30
pBAD30
pjQ200
pjQ200
pjQ200
The "poured" and "streaked" designations refer to how the transformed Shewy were put onto the plate. When transformed Shewy were streaked onto the plate, 5 microliters were used. When transformed Shewy were poured onto the plate, 100 microliters were used.
