From 2007.igem.org
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Composite part of RBS (pBca1101-I716051-kan cassette) + hemC
Digest pBca1101-I716051 (EcoR1/BamH1, 2904+1065, S)
Sub into pBca9145-I716154 (EcoR1/Bg1II, 3011+9, L)
Product is pBca9145-I716158