From 2007.igem.org

making I716025 - VHb vitreoscilla hemoglobin basic part
NdeI/BglII petDUET1 style

[pcr] AY017/18 onto pRED2   (460 bp, NdeI/BglII)
put it into petDUET1
you get I716025

AY017 // catggCATatgttagaccagcaaacc
AY018 // gtacaAGATCTttattcaaccgcttgagcg