From 2007.igem.org

Biobrickificreation of pI716101 (Low Copy ampR Plasmid - Produces RFP)
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Digest pBca9145-Bca1144#5		(EcoRI/XhoI, 2054+919, S)
Sub into pSB4A3-I7101			(EcoRI/XhoI, 3141+1136?, L)
Product is pI716101
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No Oligos Needed