From 2007.igem.org

Method Used: dBBS

PCR ca1099F/G00101 on Part A (pBca9145-Bca1090)	(553 bp)
Digest pBca9145-Bca1090				(BsaI/BamHI, 989+1102, S)
Product is I716258
Ligate with Part B
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ca1099F – ccaaaAGATCTatgaaacgttttagtctggc
G00101 – gctagCTCGAGttaGGATCCttacttaattacaccacaggc


PCR ca998/ca1099R on Part B (pBca9145-I716253)	(3393 bp)
Digest pBca9145-I716253				(BsaI/BglII, 2512 + 777, L)
Product is I716259
Ligate with Part A
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ca998 – gtatcacgaggcagaatttcag
ca1099R – gctgataaatctggagccgg

Final product is I716260