June 13

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Experiments

  • Flow cytometry carried out.
  • pL CFP (J22202) induced with IPTG for 0,1,10,50,100,1000 uM after 5 hours in sets of three.
  • pL CFP (J22202) induced with ATc for 0,1,10,25,50,100 ng/mL after 4 hrs in sets of three.
  • pL YFP (J22203) induced with IPTG for 1000uM in three sets after 4 hrs (positive control).
  • Negative control (K12Z1) in three sets (after 5 hrs).
  • 0.01,0.1 and 1 uL of inoculated pL luxI cfp inoculated in M9 in sets of three : 
M9+ 1000 uM IPTG  (1 mL)
M9+ 50 ng/mL ATC  (1 mL)

Microscopy

  • pT LuxI CFP (J22241) induced with aTc at 0,10,100 ng/mL and imaging done after 6,8,10 hrs.
  • K12Z1 induced with aTc at 0,10,100 ng/mL and imaging done after 6,8,10 hrs.


Note: There was a problem in the Flow Cytometer (FACS) sheath pressure and the filters were found misaligned. Thus fluorescence readings were not proper and we would take the FACS readings again.

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