From 2007.igem.org

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Experiments

• pLac yfp and pLac luxI cfp could not be imaged because the OD was found to be greater than 1.

• pLac yfp inoculated in 3ml LB+3ul amp and K12Z1 in 3ml LB+3ul spec (morning).

• pLac cfp Inoculated in 3ml LB+3ul amp and K12Z1 in 3ml LB+3ul spec (evening).

• The new constructs viz. j22261,j22271,j22281,j22301 were transformed.

• pLac yfp induced with various conc of IPTG (0,1,10,50,100,1000 uM).

• pLac cfp induced with various conc of IPTG (0,1,10,50,100,1000 uM).

• For FACS recalibration another set of pLac cfp was induced with IPTG levels of 0,10,100,1000 uM.