From 2007.igem.org

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Contents 1 Preparation of Agar Plates 2 Uses 3 Ingedients: 4 Method: 5 Equipment:

Preparation of Agar Plates

Uses

• Plating out and separating bacterial colonies

Ingedients:

• LB
• Ampicillin
• Agar
• Petri Dishes

Method:

1. Add 1.4g of Agar per 100mL of LB
2. Autoclave (just enough to melt)
3. Cool down to about 40-50 degrees C on bench/cold room/water.
4. Add 1/1000 volume of Ampicillin.
5. Mix and pour into petri dishes. 100ml makes 3-4 plates.
6. Leave plates to dry on bench/laminar flow partly covered.
7. Keep in cold room till used

Equipment:

• Autoclave or Microwave