Template:BerkiGEM2007 AustinSequencingFiles

From 2007.igem.org

The numbers start at where the Arkin Wiki left off.

SampleDatePlasmidClonePrimerResultFile Link
ald123 6/6/07 9145-I716053 N/A ca998 perfect ald123
ald124 6/6/07 9145-I716054 N/A ca998 perfect ald124
ald125 6/6/07 9145-I716055 #1 ca998 wrong ald125
ald126 6-7-07 9145-I716055 #2 ca998 perfect ald126
ald127 6-7-07 pBCA1101-bca9231 ca998 ald127
ald128 6-9-07 I716058 AKA:I716053m29 #1 ca998 perfect, point muation in EcoR1 Site? ald128
ald129 6-9-07 I716058 AKA:I716053m29 #2 ca998 Deletion following mutation ald129
ald130 6-9-07 I716058 AKA:I716053m29 #3 ca998 perfect, point muation in EcoR1 Site? ald130
ald131 6-9-07 I716059 AKA:I716054m108 #1 ca998 Perfect ald131
ald132 6-9-07 I716059 AKA:I716054m108 #2 ca998 Perfect ald132
ald133 6-9-07 I716059 AKA:I716054m108 #3 ca998 Perfect ald133
ald134 6-14-07 9145-Bca1154 #1 ca998 (Revised: Mixed plasmid) silent point mutation, Gln caa -> cag, %15.33 -> %28.92 ald134
ald135 6-14-07 9145-Bca1154 #2 ca998 2 point mutations, but were close calls. Probably correct ald135
ald136 6-14-07 9145-Bca1150 #1 ca998 perfect ald136
ald137 6-14-07 9145-Bca1150 #2 ca998 wrong ald137
ald138 6-14-07 pTrc-I716061 #1 ca998 wrong oligo... ald138
ald139 6-14-07 pTrc-I716061 #2 ca998 wrong oligo... ald139
ald140 6-14-07 pTrc-I716061 #3 ca998 wrong oligo... ald140
ald141 6-14-07 pTrc-I716061 #4 ca998 wrong oligo... ald141
ald142 6-14-07 9145-I716062 #1 ca998 perfect ald142
ald143 6-14-07 9145-I716062 #2 ca998 perfect ald143
ald144 6-14-07 9145-I716062 #3 ca998 wrong ald144
ald145 6-14-07 9145-I716063 #1 ca998 huge deletion ald145
ald146 6-14-07 9145-I716063 #2 ca998 huge deletion ald146
ald147 6-14-07 9145-I716063 #3 ca998 silent point mutation, T->A, Val 18.33% -> Val 10.88% ald147
ald148 6-18-07 9145-I716061 #1 D57 Perfect (shows beta HbA) ald148
ald149 6-18-07 9145-I716061 #2 D57 Perfect (shows alpha HbA) ald149
ald150 6-18-07 9145-I716061 #1 D58 Perfect ald150
ald151 6-18-07 9145-I716061 #2 D58 Perfect ald151
ald152 6-19-07 9145-1154 #3 ca998 Wrong ald152
ald153 6-19-07 9145-1154 #4 ca998 Failed ald153
ald154 6-22-07 9145-1154 #1a ca998 point mutation K96R ald154
ald155 6-22-07 9145-1154 #1b ca998 point mutation K96R ald155
ald156 6-22-07 9145-1154 #1c ca998 point mutation K96R ald156
ald157 6-26-07 9145-I716070 #1 ca998 Perfect! ald157
ald158 6-26-07 9145-I716070 #2 ca998 Perfect! ald158
ald159 6-26-07 9145-I716070 #3 ca998 Perfect! ald159
ald160 6-26-07 pTrc-I716069 #1 D57 Silent point mutation at nucleotide 103, beta chain, otherwise perfect ald160
ald161 6-26-07 pTrc-I716069 #1 D58 Silent point mutation at nucleotide 103, beta chain, otherwise perfect ald161
ald162 6-26-07 pTrc-I716069 #2 D57 Silent point mutation at nucleotide 103, beta chain, otherwise perfect ald162
ald163 6-26-07 pTrc-I716069 #2 D58 Silent point mutation at nucleotide 103, beta chain, otherwise perfect ald163
ald164 6-27-07 9145-I716073 #1 ca998 Wrong ald164
ald165 6-27-07 9145-I716073 #2 ca998 Wrong ald165
ald166 6-27-07 9145-I716073 #3 ca998 Silent point mutation! ald166
ald167 6-27-07 9145-I716072 #1 ca998 Wrong ald167
ald168 6-27-07 9145-I716072 #2 ca998 Wrong ald168
ald169 6-27-07 9145-I716072 #3 ca998 wrong ald169
ald170 6-27-07 9145-I716068 #1 ca998 Point deletion ald170
ald171 6-27-07 9145-I716068 #2 ca998 Wrong ald171
ald172 6-27-07 9145-I716068 #3 ca998 Wrong ald172
ald173 7-01-07 9145-I716068 1a ca998 Perfect ald173
ald174 7-01-07 9145-I716068 2a ca998 Point deletion, but probably not, near the end of read and it's kind of fuzzy ald174
ald175 7-01-07 9145-I716068 5a ca998 Point mutation and is parent vector from the previous step. (No mutation) ald175
ald176 7-01-07 9145-I716068 6a ca998 Perfect ald176
ald177 7-01-07 9145-I716068 8a ca998 Perfect ald177
ald178 7-04-07 9145-I716072 1 ca998 Perfect ald178
ald179 7-04-07 9145-I716072 2 ca998 Perfect ald179
ald180 7-04-07 9145-I716072 3 ca998 Perfect ald180
ald181 7-04-07 9145-I716072 4 ca998 Perfect ald181
ald182 7-04-07 9145-I716072 5 ca998 Wrong ald182
ald183 7-04-07 9145-I716072 6 ca998 Perfect ald183
ald184 7-04-07 9145-I716077 1 ca998 The point mutation changed back, still silent (Didn't "really" change back) otherwise perfect ald184
ald185 7-04-07 9145-I716077 2 ca998 Perfect until the read goes bad (probably should start sequencing backwards too) ald185
ald186 7-04-07 9145-I716078 1 ca998 Perfect ald186
ald187 7-04-07 9145-I716078 2 ca998 Perfect ald187
ald188 7-04-07 9145-I716079 1 ca998 Perfect ald188
ald189 7-04-07 9145-I716079 2 ca998 Perfect until read goes bad. ald189
ald190 07-09-07 9145-I716011 ca998 Perfect ald190
ald191 07-09-07 9145-I716011 g00101 Perfect ald191
ald192 07-09-07 9145-I716071 ca998 Wrong, missing alpha subunit ald192
ald193 07-09-07 9145-I716071 g00101 Wrong, missing alpha subunit ald193
ald194 07-09-07 9145-I716074 ca998 Wrong, missing alpha subunit ald194
ald195 07-09-07 9145-I716074 g00101 Wrong, missing alpha subunit ald195
ald196 07-09-07 9145-I716075 ca998 Wrong, missing alpha subunit ald196
ald197 07-09-07 9145-I716075 g00101 Wrong, missing alpha subunit ald197
ald198 07-09-07 9145-I716076 ca998 Wrong, missing alpha subunit ald198
ald199 07-09-07 9145-I716076 g00101 Wrong, missing alpha subunit ald199
ald200 07-09-07 9145-I716083 #2 D57 Wrong ald200
ald201 07-09-07 9145-I716084 #2 D57 Wrong construct, has providence and the 2X alpha subunit ald201
ald202 07-09-07 9145-I716085 #2 D57 Failed read: Multiple Plasmids ald202
ald203 07-09-07 9145-I716086 #2 D57 Missing Alpha Subunit ald203
ald204 07-13-07 9145-I716080 ca998 Perfect ald204
ald205 07-13-07 9145-I716081 ca998 Perfect ald205
ald206 07-13-07 9145-I716082 ca998 Silent point mutation, so ~Perfect! (Phew!) ald206
ald207 7-16-07 9145-I716074 ca998 Beta part is good, but lots of background ald207
ald208 7-16-07 9145-I716074 g00101 can see the end of an alpha, but can't tell if its a di-alpha. Terminator perfect ald208
ald209 7-16-07 9145-I716075 ca998 Can see perfect beta ald209
ald210 7-16-07 9145-I716075 g00101 Can see perfect terminator and the link between the alpha subunits, lots of "point mutations" due to a mixed template, will proceed with this ald210
ald211 7-16-07 9145-I716076 ca998 Can see beta perfectly ald211
ald212 7-16-07 9145-I716076 g00101 Term and most of alpha present. "point mutations" due to mixed read, but seems good enough to proceed ald212
ald213 7-17-07 9145-I716084 ca998 Shows promoter ald213
ald214 7-17-07 9145-I716085 ca998 Shows promoter ald214
ald215 7-17-07 9145-I716086 ca998 Shows promoter ald215
ald216 7-17-07 9145-I716071 ca998 Mixed template, but almost perfect ald216
ald217 7-17-07 9145-I716071 g00101 Mixed template, but almost perfect ald217
ald218 7-18-07 9145-I716083 ca998 Wrong, sucka. (I think I overloadded the lane, will do again) ald218
ald219 7-19-07 9145-I716084 1c ca998 Gene is gone?!! ald219
ald220 7-19-07 9145-I716084 1c g00101 Gene is gone?!! ald220
ald221 7-19-07 9145-I716085 1c ca998 Very badly mixed read ald221
ald222 7-19-07 9145-I716085 1c g00101 Very badly mixed read ald222
ald223 7-19-07 9145-I716086 1c ca998 Very badly mixed read ald223
ald224 7-19-07 9145-I716086 1c g00101 Very badly mixed read ald224
ald225 7-23-07 9145-I716083 3 ca998 Perfect ald225
ald226 7-23-07 9145-I716083 4 ca998 Perfect ald226
ald227 7-23-07 9145-I716084 1 ca998 Silent Point Mutation ald227
ald228 7-23-07 9145-I716084 3 ca998 Damn wrong ald228
ald229 7-23-07 9145-I716085 1 ca998 Point mutation! Damn it!! V->A ald229
ald230 7-23-07 9145-I716086 1 ca998 Perfecto! ald230
ald231 7-23-07 9145-I716086 3 ca998 Damn wrong ald231
ald232 7-23-07 9145-I716086 2z ca998 Perfecto! ald232
ald233 7-23-07 9145-I716083 3 g00101 Perfect ald233
ald234 7-23-07 9145-I716083 4 g00101 Perfect ald234
ald235 7-23-07 9145-I716084 1 g00101 Silent point mutation ald235
ald236 7-23-07 9145-I716084 3 g00101 Damn wrong ald236
ald237 7-23-07 9145-I716085 1 g00101 Point mutation! Damn it!! V->A ald237
ald238 7-23-07 9145-I716086 1 g00101 Perfecto! ald238
ald239 7-23-07 9145-I716086 3 g00101 Damn wrong ald239
ald240 7-23-07 9145-I716086 2z g00101 Perfecto! ald240
ald241 7-26-07 9145-I716085 #1 cpcr ca998 Read sucks, can't see link between dialpha, but still may be right if quintarabio got their act together ald241
ald242 7-26-07 9145-I716085 #2 cpcr ca998 Can see link between dialpha and whole beta, looks perfect ald242
ald243 7-26-07 9145-I716085 #1 cpcr g00101 Read sucks, can't see link between dialpha, but still may be right if quintarabio got their act together ald243
ald244 7-26-07 9145-I716085 #2 cpcr g00101 Can see link between dialpha and whole beta, looks perfect ald244
ald245 7-30-07 petDUET-I716091 1 AD0010F Perfect ald245
ald246 7-30-07 petDUET-I716091 1 AD0011F Perfect ald246
ald247 7-30-07 petDUET-I716093 2-1 AD0010F Perfect beta and up to di-alpha linkage ald247
ald248 7-30-07 petDUET-I716094 1-3 AD0010F Perfect ald248
ald249 7-30-07 petDUET-I716094 1-3 AD0011F Perfect ald249
ald250 8-14-07 petDUET-I716351 1 g00101 Shows little bit of cytochrome cassette ald250
ald251 8-14-07 petDUET-I716351 1 ca998 Shows little bit of hem cassette ald251
ald252 8-14-07 petDUET-I716353 2 AD0012F So very wrong (They said they used ca998 and g00101 though...) ald252
ald253 8-14-07 petDUET-I716353 2 AD0012R So very wrong (They said they used ca998 and g00101 though...) ald253
ald254 8-15-07 petDUET-I716092 3 ca998 Wrong oligos...my bad ald254
ald255 8-15-07 petDUET-I716092 3 g00101 Wrong oligos...my bad ald255
ald256 8-22-07 petDUET-I716353 #1 AD0016R Can see the beginning of ADHE! But there is a 5 base pair insertion between the rbs and the ORF ald256
ald257 8-22-07 petDUET-I716353 #2 AD0016R Can see the beginning of ADHE! ald257
ald258 8-22-07 9145-I716354 2-1 #2 ca998 Perfect beta ald258
ald259 8-22-07 9145-I716354 2-1 #2 g00101 Perfect alpha ald259
ald260 8-22-07 9145-I716356 1-2 ca998 Perfect beta and beginning of di alpha ald260
ald261 8-22-07 9145-I716356 1-2 g00101 Can see dialpha linkage. Point mutation (that I called earlier) "changed back", silent mutation ald261
ald262 8-22-07 9145-I716357 1-1 ca998 Bad read: Can only see beginning of beta. Looks good though. ald262
ald263 8-22-07 9145-I716357 1-1 g00101 Perfect alpha, can see end of beta ald263
ald264 8-22-07 9145-I716357 2-1 ca998 Questionable point mutation ald264
ald265 8-22-07 9145-I716357 2-1 g00101 Questionable point mutation ald265
ald266 8-22-07 petDUET-I716092 3 AD0010F Beta is there, really bad read though ald266
ald267 8-22-07 petDUET-I716092 3 AD0011R di-alpha is there, very bad read. ald267
ald268 9-7-07 I716366 1 ca998 Perfect ald268
ald269 9-7-07 I716367 1 ca998 Perfect ald269
ald270 9-17-07 I716362 1 g00101 sodc/katg are present ald270
ald271 9-17-07 I716364 1 g00101 sodc/katg are present ald271
ald272 9-21-07 I716368 2 g00101 Cytochromes present ald272
ald273 9-21-07 I716369 2 g00101 Cytochromes present ald273
ald274 9-21-07 T7-HemABCD-TT 3 g00101 Terminator Present ald274
ald275 10-3-07 I716276-277 1 g00101 Wrong, no AHSP ald275
ald276 10-3-07 I716269-TT 1 g00101 Can see TT-SodC-Ending of KatG ald276
ald277 10-3-07 I716378 3 AD0011F Read goes bad too quick, but can see bits and pieces which match to the AHSP in correct position ald277
ald278 10-11-07 I716379 2 g00101 Perfect AHSP addition ald278
ald279 10-11-07 I716376 1 g00101 good ald279
ald280 10-11-07 I716377 3 g00101 good ald280
ald281 10-11-07 I716380+276+277 3 g00101 only metAP addition (only 277) ald281
ald282 10-11-07 I716381+276+277 3 g00101 onlt metAP addition (only 277) ald282
ald283 ald283
ald284 ald284
ald285 ald285
ald286 ald286
ald287 ald287
ald288 ald288
ald289 ald289
ald290 ald290
ald291 ald291
ald292 ald292
ald293 ald293
ald294 ald294
ald295 ald295
ald296 ald296
ald297 ald297
ald298 ald298
ald299 ald299
ald300 ald300
ald301 ald301
ald302 ald302
ald303 ald303
ald304 ald304
ald305 ald305
ald306 ald306
ald307 ald307
ald308 ald308
ald309 ald309
ald310 ald310
ald311 ald311
ald312 ald312
ald313 ald313
ald314 ald314
ald315 ald315
ald316 ald316
ald317 ald317
ald318 ald318
ald319 ald319
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