From 2007.igem.org

|otsAF||06/29/07 ||pBca9145-Bca1144||#1||ca998||Great no problems with forward until after 900bp. Insert was completely present.|| otsAF

|otsAR||06/29/07 ||pBca9145-Bca1144||#2||go1001||Full insert with only problem is a 6 bp sequence after the XhoI site that I heard others have had problems with. Insert was completely present.|| otsAR

|otsBF||06/29/07 ||pBca9145-Bca1144||#3||ca998||The forward primer completely sequenced the insert except for one point mutation that was after the stop codon and before the BamHI site. Insert was completely present.|| otsBF

|otsBR||06/29/07 ||pBca9145-Bca1144||#4||go1001||Took care of the one point mutation. Insert was completely present.|| otsBR

|otsBAF||06/29/07 ||pBca9145-Bca1144||#1||ca998||Covers most of otsB and covers point mutation. Insert was not completely present.|| otsBAF

|otsBAAF||06/29/07 ||pBca9145-Bca1144||#2||otsAF||Covers the middle of otsA to the end. Insert was not completely present.|| otsBAAF

|otsBABF||06/29/07 ||pBca9145-Bca1144||#3||otsBF||Covers from middle of otsB to middle of otsA. Insert was not completely present.|| otsBABF

|otsBAR||06/29/07 ||pBca9145-Bca1144||#4||go1001||Covers from the end to almost beginning of otsA. Insert was not completely present.|| otsBAR