Week 15

From 2007.igem.org

10/08/07
  • We inoculate:
-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
-E.Coli;
-[http://partsregistry.org/Part:BBa_I763032 I763032] (high copy plasmid).
  • Digestion of:
-[http://partsregistry.org/Part:BBa_I763035 I763035] with Eco/Pst1;
-[http://partsregistry.org/Part:BBa_I763007 I763007] with Eco/spe;
-[http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Xba;
-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Spe/Xba.
  • Ligation for:
-[http://partsregistry.org/Part:BBa_I763035 I763035]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-[http://partsregistry.org/Part:BBa_I763007 I763007]+[http://partsregistry.org/Part:BBa_I763026 I763026];
-[http://partsregistry.org/Part:BBa_I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
  • Run on electrophoresis gel.
  • [http://partsregistry.org/Part:BBa_I763007 I763007] and [http://partsregistry.org/Part:BBa_I763026 I763026] band extraction.
  • O/N ligation for:
-[http://partsregistry.org/Part:BBa_I763026 I763026]+[http://partsregistry.org/Part:BBa_I763007 I763007];
-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-[http://partsregistry.org/Part:BBa_I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
  • We inoculate:
-[http://partsregistry.org/Part:BBa_I763031 I763031](low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763016 I763016](low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763032 I763032] (high copy plasmid).


10/09/07
  • Transformation of:
-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007];
-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763032 I763032] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid).
  • We inoculate in 6ml:
-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid).
  • Ligation for:
-[http://partsregistry.org/Part:BBa_I763016 I763016] (Eco/Spe)+GFP.(Eco/Xba).


10/10/07
  • Miniprep of:
-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid).
  • Digestion for :
-[http://partsregistry.org/Part:BBa_I763031 I763031] with Xba/Pst1;
-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid) with Spe/Pst1;
  • Run on electrophoresis gel and [http://partsregistry.org/Part:I763016 I763016] band extraction.
  • Ligation for :
-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid)+GFP;
-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:BBa_C0012 C0012] (high copy plasmid).
  • We inoculate in 6ml:
-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007](high copy plasmid);
-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid).
10/11/07
  • Stock preparation (500ul) of:
-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007](high copy plasmid);
-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid).
  • Miniprep of:
-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763007 I763007](high copy plasmid);
-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid).
  • Fluorescence test of:
-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007] (high copy plasmid);
-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid).
  • Control digestion of:
-[http://partsregistry.org/Part:BBa_I763031 I763031] with Xba/Pst1;
-[http://partsregistry.org/Part:BBa_I763016 I763016] with Xba/Pst1 and Spe/Xba;
-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007] with Xba/Pst1 and Spe/Xba;
-[http://partsregistry.org/Part:BBa_I763024 I763024] with Xba/Pst1;
-[http://partsregistry.org/Part:BBa_I763007 I763007] with Xba/Pst1.
  • Run on electrophoresis gel and band extraction.
  • Transformation of:
-[http://partsregistry.org/Part:BBa_I763019 I763019];
-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:BBa_C0012 C0012];
-[http://partsregistry.org/Part:BBa_I763032 I763032].
  • We inoculate
-[http://partsregistry.org/Part:BBa_I763019 I763019].
10/12/07
  • Miniprep of:
-[http://partsregistry.org/Part:BBa_I763019 I763019].
  • Digestion of:
-[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Pst1;
-[http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba.
  • Run on electrophoresis gel.
  • [http://partsregistry.org/Part:BBa_I763019 I763019] (Xba/Pst1) and [http://partsregistry.org/Part:BBa_I763019 I763019] (Eco/Xba) band extraction.


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