Wisconsin/PCR
From 2007.igem.org
Primer Stock
First check primer concentration using spectrophotometer... then prepare stock of 500uL 10uM.
Basic Non-Sequencing PCR
- 38.5uL H2O
- 5uL 10x ExTaq buffer
- 1uL 10mM dNTPs
- 1uL Forward Primer
- 1uL Reverse Primer
- 1uL Template
- 0.5uL ExTaq (add this last)
- 2uL MgCl2 25mM
Run on Ansari PCR machine CDC-02
PCR Purification
Follow instruction in the [http://www1.qiagen.com/literature/protocols/QIAquickSpin.aspx QIAquick Spin Handbook]
