Alberta/Calender/August

From 2007.igem.org

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(August 3)
(August 3)
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<b> Meeting @ 1700hrs</b>
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<b> Meeting @ 1700hrs</b><br>
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[[Alberta/Calender/August#August|to the top]]
 
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<b>Captain's Log, Stardate: 080307-1153</b>
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<b>Captain's Log, Stardate: 080307-1153</b> <br>
Minipreps of lifeforms Buddy and Betty <br>
Minipreps of lifeforms Buddy and Betty <br>
Not enough room in box with blue tape, designed a new holding vessel (i.e. box with white tape below box with blue tape in the freezer) for minipreps of aforementioned lifeforms.<br>
Not enough room in box with blue tape, designed a new holding vessel (i.e. box with white tape below box with blue tape in the freezer) for minipreps of aforementioned lifeforms.<br>
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[[Alberta/Calender/August#August|to the top]]
== August 4 ==
== August 4 ==

Revision as of 22:31, 3 August 2007

Contents

August

August 2007
Su M Tu W Th F Sa
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31


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August 1

Schedule: CZ,NG,VH

General Notes:
1. Run gel of B0034 SpeI/PstI double digest; extract gel with QIAgen kit; stored in freezer.
2. PCR for
a. Enny + B0034 miniprep 1 to 8
b. Betty colonies (10)
c. Buddy colonies (10)
Follow James' protocol. Also see blackboard for James' comment about PCR.

For tomorrow
1. run gel of PCR prodcut which are in the thermocycler �
2. check restreaked single colonies of the Betty and Buddy colony PCR
3. grow up Betty and Buddy overnight and for miniprep and ligation later
4. Make Amp plates (we only have 4 more)
5. Autoclave test tubes

Quotes of the day: We are tired.

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August 2

Modeling Meeting @ 1830hrs in CAB373
General Meeting @ 1900hrs in CAB373
Party afterward's @ Garneau pub

Schedule:

MC, JP & AF are meeting at 1700hrs to get some stuff done before the meeting happens; come join in the fun if you'd like.

Lab Notes:

Made AMP plates
Autoclaved test tubes
Ran gel of ?
Started O/N of Buddy & Betty
Checked restreaked colonies of Buddy & Betty --> positive growth except for 9&10 but this is expected because they are controls

James did a loving PCR of Benny in B0034

-MC, ED, JP, JB


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August 3

Schedule:

Nik cant make it he just isn't crazy enough looks like justin is currently the only one crazy enough
Michelle & Justin are now the long weekend crew - be prepared for ILLUSIONS!!!! or perhaps just some awesome pipetting techniques while they're prepping to champ out at the ULTIMATE pipetting Olympiads.


Meeting @ 1700hrs


Captain's Log, Stardate: 080307-1153

Minipreps of lifeforms Buddy and Betty
Not enough room in box with blue tape, designed a new holding vessel (i.e. box with white tape below box with blue tape in the freezer) for minipreps of aforementioned lifeforms.
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August 4

Meeting at 1000hrs - Michelle will definately be there at 1000 Justin is expected at 1030


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August 5

Meeting at 1000hrs - Michelle will definately be there at 1000 Justin is expected at 1030

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August 6

Meeting at 1000hrs - Michelle will definately be there at 1000 Justin is expected at 1030

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August 7

Schedule:

ED,VH,AF


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August 8

Schedule:

JP,NK,MC


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August 9

Schedule:

ED,AF,JG

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August 10

Schedule:

CZ, MC, JG

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August 11

Schedule:

JP, AL, NK

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August 12

Schedule:

CZ, AL, NK


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August 13

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August 14

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August 15

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August 16

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August 17

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August 18

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August 19

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August 20

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August 21

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August 22

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August 23

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August 24

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August 25

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August 26

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August 27

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August 28

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August 29

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August 30

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August 31

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