Alberta/Calender/August

From 2007.igem.org

< Alberta | Calender
Revision as of 01:38, 7 August 2007 by Mcchan (Talk | contribs)

Contents

August

August 2007
Su M Tu W Th F Sa
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31


To July 2007
To September 2007
Back to UofA iGEM Home

August 1

Schedule: CZ,NG,VH

General Notes:
1. Run gel of B0034 SpeI/PstI double digest; extract gel with QIAgen kit; stored in freezer.
2. PCR for
a. Enny + B0034 miniprep 1 to 8
b. Betty colonies (10)
c. Buddy colonies (10)
Follow James' protocol. Also see blackboard for James' comment about PCR.

For tomorrow
1. run gel of PCR prodcut which are in the thermocycler �
2. check restreaked single colonies of the Betty and Buddy colony PCR
3. grow up Betty and Buddy overnight and for miniprep and ligation later
4. Make Amp plates (we only have 4 more)
5. Autoclave test tubes

Quotes of the day: We are tired.

to the top

August 2

Modeling Meeting @ 1830hrs in CAB373
General Meeting @ 1900hrs in CAB373
Party afterward's @ Garneau pub

Schedule:

MC, JP & AF are meeting at 1700hrs to get some stuff done before the meeting happens; come join in the fun if you'd like.

Lab Notes:

Made AMP plates
Autoclaved test tubes
Ran gel of ?
Started O/N of Buddy & Betty
Checked restreaked colonies of Buddy & Betty --> positive growth except for 9&10 but this is expected because they are controls

James did a loving PCR of Benny in B0034

-MC, ED, JP, JB


to the top

August 3

Schedule:

Nik cant make it he just isn't crazy enough looks like justin is currently the only one crazy enough
Michelle & Justin are now the long weekend crew - be prepared for ILLUSIONS!!!! or perhaps just some awesome pipetting techniques while they're prepping to champ out at the ULTIMATE pipetting Olympiads.


Meeting @ 1700hrs

Captain's Log, Stardate: 080307-1153

Minipreps of lifeforms Buddy and Betty
Not enough room in box with blue tape, designed a new holding vessel (i.e. box with white tape below box with blue tape in the freezer) for minipreps of aforementioned lifeforms.
-JB

Made gel
Digested (double) Buddy & Betty minipreps
Digest (single) I0500 - from tube we believe is a miniprep in the -20 freezer
weekend to-do list
-JP, ML, MC


to the top

August 4

Meeting at 1000hrs - Michelle will definately be there at 1000 Justin is expected at 1030


Lab Notes:

Made a project timeline - check out the cabinets on the left side of the 4C-degree fridge
Ran gel - no bands for I0500 (we were sad) but there are awesome bands of Betty & Buddy
Attempting to figure out the I0500 problem; transformed into XL10 Gold & HB101

-MC, JP, ML

to the top

August 5

Meeting at 1000hrs - Michelle will definately be there at 1000 Justin is expected at 1030 Lab Notes:

1-transform I0500 into HB101 and XL10 gold
2-restricted mini's of Buddy and Betty with Xba/Pst (confirmed with gel their presence yesterday)
3-ran I0500 samples in gel and no DNA present, chucked out samples - re transform...

For Tomorrow

1-gel purify Buddy and Betty
2-ligate Buddy and Betty into Boo34 (overnite)
3-start ONs of I0500 for mini on tuesday

to the top

August 6

Meeting at 1000hrs - Michelle will definately be there at 1000 Justin is expected at 1030

to the top

August 7

Schedule:

Justin, can you trade me for Wednesday, please???? I will owe you big. -ED

ED,VH,AF


to the top

August 8

Schedule:

JP,NK,MC


to the top

August 9

Schedule:

ED,AF,JG

to the top

August 10

Schedule:

CZ, MC, JG

to the top

August 11

Schedule:

JP, AL, NK

to the top

August 12

Schedule:

CZ, AL, NK


to the top

August 13

to the top

August 14

to the top

August 15

to the top

August 16

to the top

August 17

to the top

August 18

to the top

August 19

to the top

August 20

to the top

August 21

to the top

August 22

to the top

August 23

to the top

August 24

to the top

August 25

to the top

August 26

to the top

August 27

to the top

August 28

to the top

August 29

to the top

August 30

to the top


August 31

to the top


UofA iGEM Home
To July 2007
To September 2007