Alberta/Calender/August

From 2007.igem.org

Contents

August

August 2007
Su M Tu W Th F Sa
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31


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August 1

Schedule: CZ,NG,VH

General Notes:
1. Run gel of B0034 SpeI/PstI double digest; extract gel with QIAgen kit; stored in freezer.
2. PCR for
a. Enny + B0034 miniprep 1 to 8
b. Betty colonies (10)
c. Buddy colonies (10)
Follow James' protocol. Also see blackboard for James' comment about PCR.

For tomorrow
1. run gel of PCR prodcut which are in the thermocycler �
2. check restreaked single colonies of the Betty and Buddy colony PCR
3. grow up Betty and Buddy overnight and for miniprep and ligation later
4. Make Amp plates (we only have 4 more)
5. Autoclave test tubes

Quotes of the day: We are tired.

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August 2

Modeling Meeting @ 1830hrs in CAB373
General Meeting @ 1900hrs in CAB373
Party afterward's @ Garneau pub

Schedule:

MC, JP & AF are meeting at 1700hrs to get some stuff done before the meeting happens; come join in the fun if you'd like.

Lab Notes:

Made AMP plates
Autoclaved test tubes
Ran gel of ?
Started O/N of Buddy & Betty
Checked restreaked colonies of Buddy & Betty --> positive growth except for 9&10 but this is expected because they are controls

James did a loving PCR of Benny in B0034

-MC, ED, JP, JB


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August 3

Schedule:

Nik cant make it he just isn't crazy enough looks like justin is currently the only one crazy enough
Michelle & Justin are now the long weekend crew - be prepared for ILLUSIONS!!!! or perhaps just some awesome pipetting techniques while they're prepping to champ out at the ULTIMATE pipetting Olympiads.


Meeting @ 1700hrs

Captain's Log, Stardate: 080307-1153

Minipreps of lifeforms Buddy and Betty
Not enough room in box with blue tape, designed a new holding vessel (i.e. box with white tape below box with blue tape in the freezer) for minipreps of aforementioned lifeforms.
-JB

Made gel
Digested (double) Buddy & Betty minipreps
Digest (single) I0500 - from tube we believe is a miniprep in the -20 freezer
weekend to-do list
-JP, ML, MC


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August 4

Meeting at 1000hrs - Michelle will definately be there at 1000 Justin is expected at 1030


Lab Notes:

Made a project timeline - check out the cabinets on the left side of the 4C-degree fridge
Ran gel - no bands for I0500 (we were sad) but there are awesome bands of Betty & Buddy
Attempting to figure out the I0500 problem; transformed into XL10 Gold & HB101

-MC, JP, ML

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August 5

Meeting at 1000hrs - Michelle will definately be there at 1000 Justin is expected at 1030 Lab Notes:

1-transform I0500 into HB101 and XL10 gold
2-restricted mini's of Buddy and Betty with Xba/Pst (confirmed with gel their presence yesterday)
3-ran I0500 samples in gel and no DNA present, chucked out samples - re transform...
-MC, JP & ML (in spirit)

For Tomorrow

1-gel purify Buddy and Betty
2-ligate Buddy and Betty into Boo34 (overnite)
3-start ONs of I0500 for mini on tuesday

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August 6

Meeting at 1000hrs - Michelle will definately be there at 1000 Justin is expected at 1030

Lab Notes:

- JP, ML, AF, MC

Notes:

1- re-plated I0500 tranformations on Kan and AMP plates - hope for growth
2- ran buddy and betty XbaI/PstI restriction products on gel and gel extracted
3- began ligation reaction with B0034 (in back shaker at 13 degrees)
4- started making butanol plates...but it might not work so well with higher percentages because when you plate the agar its warm/hot and when you add the butanol it bubbles like mad (evaporates). We tried pouring at lower temp, but it prooved disasterous

Fact of the Day

A good ligation is like a hoola-hoop, they're both circular and only one is methylated

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August 7

Schedule:

JP,VH,AF


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August 8

Schedule:

ED,NK,MC


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August 9

Schedule:

ED,AF,JG

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August 10

Schedule:

CZ, MC, JG

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August 11

Schedule:

JP, AL, NK

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August 12

Schedule:

CZ, AL, NK


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August 13

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August 14

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August 15

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August 16

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August 17

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August 18

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August 19

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August 20

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August 21

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August 22

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August 23

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August 24

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August 25

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August 26

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August 27

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August 28

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August 29

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August 30

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August 31

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