Alberta/Calender/July

From 2007.igem.org

(Difference between revisions)
(July 14)
(July 14)
Line 220: Line 220:
== July 14 ==
== July 14 ==
 +
<b>Schedule:</b>
 +
 +
AL,JP,NK
<b>General Notes:</b>
<b>General Notes:</b>

Revision as of 01:57, 14 July 2007

Contents

July

July 2007
Su M Tu W Th F Sa
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31


To August 2007
Back to UofA iGEM Home

July 1

to the top

July 2

to the top

July 3

to the top

July 4

Lab Notes:

Transformations of: J61003 (vector containing GFP), J23119 (constitutive promotor), E1010 (RFP), J45100 (methyl salicylate), 0034 (RBS). Transform into XL10 gold cells. Plate on Amp.

-ED, JG, ML, MC, VH, AL, CZ

to the top

July 5

to the top

July 6

Lab Notes:

Transformation of E1010 did not work, it is in a Kan vector. SMRT.

Set up 4X5mL overnights of pSB1A3, J45100, B0034, J61003.

-ED

to the top

July 7

Lab Notes:

Glycerol stocks of overnights from July 6. Stored in -80 freezer. Miniprep from overnights started July 6. Stored in "iGEM" freezer box in -20 freezer.

-JP,JB,AL

to the top

July 8

Lab Notes:

Transform E1010 and A0500 into XL10 Gold. Plate on Kan.

Pour Kan and Amp plates. Make TBE and TAE buffer.

-ED, JP, MC, VH, CZ

to the top

July 9

Lab Notes:

Retransform E1010 and A0050 into XL10Gold cells using new plates that were poured Sunday.

-ED, VH, JG

to the top

July 10

Lab Notes:

Transformations of E1010 and A0050 were not successful. Tomorrow we will try with a new cell line and enriched media.

-ED, MC, AF, CZ

to the top

July 11

Lab Notes:

Third attempt at transforming the Kan resistant BioBricks. Transformed E1010, A0500, and pSB2K3 into XL10 Gold, XL10 Gold with enriched media (Magnesium & Glucose), and DH5a cells. Plated 100uL and the pellet remaining after taking the 100uL aliquot on Kan plates.

Restriction digest of J61003 with Xba and Spe. Run on 0.8% agarose gel. Cut out 2290 band, will gel extract tomorrow.

-ED, MC, NG, JG, VH, CZ

to the top

July 12

General Notes:

Calender up and running.

Meeting tonight at 7:00 in CSC 2-49.

Received Chlorobium tepidum, meeting with Dr. Jeff Fuller (with Capital Health) to discuss the use of their anaerobic chambers!! -Justin

Lab Notes:

Transformations with DH5a cells worked, but we will need to select for single colonies tonight.

Streak for single colonies of E1010, A0500, J61003.

Purification of gel slice of digested J61003.

-ED, JG, AL, JB, MC, CZ

to the top

July 13

General Notes:

Everyone who is coming tonight please be present 7:00pm sharp

Lab Notes:

Started 4X5mL overnights of E1010 and I0050 at 8:30 am. Should be ready for miniprep tonight.

-ED, JB

No growth - made some Kan plates

-MC, JP, JG, CZ

to the top

July 14

Schedule:

AL,JP,NK

General Notes:

If you are scheduled during the day please be in the lab at 1230hrs (PM) Don't forget that the Bio-Sci doors lock at 7:00 so if you are trying to get in after 1900hrs call 492-2911

to the top

July 15

to the top

July 16

to the top

July 17

to the top

July 18

to the top

July 19

Meeting at 7:00, Room TBA.


to the top

July 20

to the top

July 21

to the top

July 22

to the top

July 23

to the top

July 24

to the top

July 25

to the top

July 26

Meeting at 7:00, Room TBA.


to the top

July 27

to the top

July 28

to the top

July 29

to the top

July 30

to the top


July 31

to the top


UofA iGEM Home
To June 2007
To August 2007