Alberta/Calender/September

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Contents

September

September 2007
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== September 1 ==

Lab Notes
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later

-CZ


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September 2

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September 3

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September 4

Schedule:

JP,ML,JG 7:00pm


For the record:
Buddy = BuOH DH 1235 bp
Betty = Bb-bhBu-coa dh 923 bp
Benny = Butyryl coa dh 1184 bp
enny = Enoyl coa Hydratase 860 bp
Diezel Blaze = buAld-Dh 2639 bp

Notes:
Moving was done this mid-day.
New location M349
JG has key & access
- MC, ED, JG

Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC

Night Crew

-JP, JG

Set up new lab

Things we need - Water Bath, Scale, disposable 13ml overnight tubes, Tip waste

lab work

Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab

To Do list for tomorrow

- Restriction on DB needs to be done

- If I0500 comes in from calgary it needs to be transformed.

- Transform R0800 which is a back up promoter just in case

-start restriction on BE and BB for SPE and PST

-Note we also have to do sequenceing on BB and BE

Shout out to calgary for saving our necks Shout out to the move in crew to the top

September 5

Schedule:

NG,JP,ML 7:00pm


MC will be in AM (JG - what time will you be in because you have the key?) thnx - MC

JG can get up as early as you would like lets say 8:00 (if you are willing to get up earlier just let me know)


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September 6

Schedule:

NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)


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September 7

Schedule:

MC, JG 8:00 AM ... If you would like to meet later michelle just let me know i am free untill 11:00am But i only have one lecture that day any time else is good

I don't have class all day - so whenever is good for you - MC

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September 8

Schedule:

NG AL JP Noon?

I'm good to go at 1:15pm -jp

I can be there at 11am - al

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September 9

Schedule:

JG ED NG Noon?

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September 10

Schedule:


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September 11

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September 12

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September 13

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September 14

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September 15

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September 16

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September 17

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September 18

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September 19

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September 20

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September 21

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September 22

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September 23

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September 24

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September 25

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September 26

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September 27

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September 28

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September 29

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September 30

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