Arthur Yu Notebook

From 2007.igem.org

(Difference between revisions)
(6/4 Training Finishes, Real Stuff Starts)
(6/4 Training Finishes, Real Stuff Starts)
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* Colony PCR'd the incubated E.coli
* Colony PCR'd the incubated E.coli
* Ran gel of the digest + PCR
* Ran gel of the digest + PCR
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* PICTURE: [[Image:060407gelayu.jpg|200px|right]]
+
[[Image:060407gelayu.jpg|200px|right]]
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* ^ PCR product / Miniprep 1 / Parent Plasmid / Miniprep 2 / Ladder
+
* >>> PCR product / Miniprep 1 / Parent Plasmid / Miniprep 2 / Ladder >>>
* No bands for PCR or parent. Confused? Other ones look great.
* No bands for PCR or parent. Confused? Other ones look great.
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<br>

Revision as of 00:33, 5 June 2007

My Construction Files
My Sequencing Files


6/4 Training Finishes, Real Stuff Starts

  • Incubated some colonies
  • Miniprep'd already-been-incubated colonies (2)
  • Double digest of the 2 minipreps + parent plasmid
  • Colony PCR'd the incubated E.coli
  • Ran gel of the digest + PCR
060407gelayu.jpg
  • >>> PCR product / Miniprep 1 / Parent Plasmid / Miniprep 2 / Ladder >>>
  • No bands for PCR or parent. Confused? Other ones look great.


As for me: Wiki acc works now.
Designing oligos and will compare with Vai. Ayu 18:19, 4 June 2007 (EDT)

to do