http://2007.igem.org/wiki/index.php?title=Berkeley_LBL/DNAGelElectrophoresis&feed=atom&action=historyBerkeley LBL/DNAGelElectrophoresis - Revision history2024-03-28T22:25:27ZRevision history for this page on the wikiMediaWiki 1.16.5http://2007.igem.org/wiki/index.php?title=Berkeley_LBL/DNAGelElectrophoresis&diff=47828&oldid=prevJoyxi at 03:44, 27 October 20072007-10-27T03:44:34Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:44, 27 October 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>242 g Tris Base</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>242 g Tris Base</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>57.1 mL Glacial Acetic Acid</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>57.1 mL Glacial Acetic Acid</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>600 mL ddH2O</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>600 mL ddH2O</div></td></tr>
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</table>Joyxihttp://2007.igem.org/wiki/index.php?title=Berkeley_LBL/DNAGelElectrophoresis&diff=47810&oldid=prevJoyxi at 03:42, 27 October 20072007-10-27T03:42:27Z<p></p>
<p><b>New page</b></p><div>1. Prepare 50X TAE as:<br />
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242 g Tris Base<br />
57.1 mL Glacial Acetic Acid<br />
600 mL ddH2O<br />
Mix. Bring volume to 1 L. <br />
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2. Make o.8% Agarose gel<br />
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3. Melt agarose in the microwave<br />
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4. Seal horizontal gel apparatus. Pour molten agarose onto gel plate to a depth of 4 - 8 mm.<br />
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5. Insert a comb until its base is 1 mm from the base of the gel. Allow to cool<br />
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6. Remove comb and submerge in 1X TAE buffer.<br />
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7. Prepare 6X Loading Dye as.<br />
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8. Add 1/5 volume 6X Loading Dye to sample. Mix well.<br />
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9. Add sample to well. Do not overflow well. For large samples, either reduce sample in volume prior to adding dye, or load into multiple wells.<br />
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10. Electrophorese until Bromophenol Blue is near the end of the gel. This dye runs at ~800 b.<br />
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11. Remove gel and visualize bands under uv light.</div>Joyxi