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[[Nhu Nguyen Notebook]]<br>
[[Nhu Nguyen Notebook]]<br>
[[Hannah Cole Notebook]]<br>
[[Hannah Cole Notebook]]<br>
[[BerkiGEM2007Setup Setup]]<br>

Revision as of 00:26, 5 September 2007

Berkeley BactobloodHeader.jpg

The global demand and importance for cheap, available, and disease free blood substitutes is undisputed. There are currently no red blood cell substitutes approved for clinical use in the US or the UK, and whole blood is almost always in short supply. Underdeveloped countries that need blood the most simply don’t have the infrastructure to support donation and storage, in addition a sizeable fraction of the population are disease carriers.

We have developed an innovative and cheap red blood cell substitute by modifying the E. coli chassis to make it safer to inject into the human bloodstream, and by adding components for oxygen delivery. A modified lipopolysaccharide should significantly (1000-10000x) reduce sepsis activity in the human bloodstream. A hemoglobin mutated to increase OD50 to match that of natural human blood cells after diphosphoglycerase. Heme and cytochrome B5 and B5 reductase complement the hemoglobin. Additional chaperone proteins such as sodC, HPI-katG, map, and AHSP were added to increase hardiness and prolong the half-life of the E. coli in the bloodstream. We are also investigating myoglobin, and potential freeze-drying to preserve E. coli, with OtsA, OtsB, thpA through D (trihelose - ots) (hydroxyectoine).

Team Members

John Dueber
Christopher Anderson
Adam Arkin
Jay Keasling

Teaching Assistants
Farnaz Nowroozi
Amin Hajimorad
Rickey Bonds

Undergraduate Researchers
Arthur Yu
Austin Day
David Tulga
Kristin Doan
Samantha Liang
Vaibhavi Umesh
Kristin Fuller

High School Students
Vincent Parker
Nhu Nguyen
Hannah Cole

Team Resources

Oligo List Spreadsheet
CloneSaver Spreadsheet
Our BioBrick Parts
All construction files
All sequencing files

If you need an invitation to the spreadsheets, ask Sam.

Tools and Guides

Biobricks and Cloning Tutorials
Pairwise Alignment Online
Multiple Sequence Alignment
Wiki Formatting Guide

Useful Links

UC Berkeley iGEM 2006 OpenWetWare
UC Berkeley iGEM 2006 wiki
iGEM wikis: 2006, 2007
Registry of Standard Biological Parts
Biobricks Parts Lists: 2005, 2006, 2007

Team Notebooks

John Dueber Notebook
Christopher Anderson Notebook
Farnaz Nowroozi Notebook
Amin Hajimorad Notebook
Rickey Bonds Notebook

Keep your wiki notebooks, sequencing/construction logs, and the registry updated!

Arthur Yu's 1337 Notebook
Austin Day Notebook
David Tulga's Notebook
Kristin Doan Notebook
Samantha's Notebook (June - July 19, 2007
Samantha's Notebook (July 20, 2007 - present)
Vaibhavi Umesh Notebook
Kristin Fuller Notebook

Vincent Parker Notebook
Nhu Nguyen Notebook
Hannah Cole Notebook
BerkiGEM2007Setup Setup