BerkiGEM2007-ArthurConstructionFile12

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construct ptet/rbs/wbbL/rbs/neuS in lo copy

digest I716101 low copy guy (EcoRI, BamHI), 3150+910, largest
digest I716008 ptet/rbs/wbbL/rbs/neuS (EcoRI, BamHI, AlwNI) 2146+1510+553, largest
Ligate them prod I716012 ptet/rbs/wbbL/rbs/neuS in lo copy