BerkiGEM2007-ArthurConstructionFile13
From 2007.igem.org
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<pre> | <pre> | ||
- | construct | + | construct ptet/rbs/wbbL/rbs/neuS in lo copy |
- | digest | + | digest I716101 low copy guy (EcoRI, BamHI), 3150+910, largest |
- | digest | + | digest I716008 ptet/rbs/wbbL/rbs/neuS (EcoRI, BamHI, AlwNI) 2146+1510+553, largest |
- | Ligate them | + | Ligate them prod I716012 ptet/rbs/wbbL/rbs/neuS in lo copy |
+ | </pre> | ||
+ | |||
+ | Another version tried... | ||
+ | |||
+ | <pre> | ||
+ | construct ptet/rbs/wbbL/rbs/neuS in lo copy | ||
+ | |||
+ | digest I716101 low copy guy (EcoRI, BamHI), 3150+910, largest | ||
+ | digest I716008 ptet/rbs/wbbL/rbs/neuS (EcoRI, BamHI, BglI) 2146+1149+914, largest | ||
+ | Ligate them prod I716012 ptet/rbs/wbbL/rbs/neuS in lo copy | ||
</pre> | </pre> |
Latest revision as of 23:28, 3 July 2007
construct ptet/rbs/wbbL/rbs/neuS in lo copy digest I716101 low copy guy (EcoRI, BamHI), 3150+910, largest digest I716008 ptet/rbs/wbbL/rbs/neuS (EcoRI, BamHI, AlwNI) 2146+1510+553, largest Ligate them prod I716012 ptet/rbs/wbbL/rbs/neuS in lo copy
Another version tried...
construct ptet/rbs/wbbL/rbs/neuS in lo copy digest I716101 low copy guy (EcoRI, BamHI), 3150+910, largest digest I716008 ptet/rbs/wbbL/rbs/neuS (EcoRI, BamHI, BglI) 2146+1149+914, largest Ligate them prod I716012 ptet/rbs/wbbL/rbs/neuS in lo copy