BerkiGEM2007-ArthurConstructionFile13

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<pre>
<pre>
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construct stuff
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construct ptet/rbs/wbbL/rbs/neuS in lo copy
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digest I716009 cm/rbs/cytB5 (EcoRI, BamHI) 2063+1236, smaller
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digest I716101 low copy guy (EcoRI, BamHI), 3150+910, largest
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digest I716010 kan/rbs/cytB5red (EcoRI, BglII)   2944+1027, larger
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digest I716008 ptet/rbs/wbbL/rbs/neuS (EcoRI, BamHI, AlwNI) 2146+1510+553, largest
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Ligate them. product I716011 cm-rbs-cytB5-rbs-cytB5red
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Ligate them prod I716012 ptet/rbs/wbbL/rbs/neuS in lo copy
</pre>
</pre>

Revision as of 23:37, 2 July 2007

construct ptet/rbs/wbbL/rbs/neuS in lo copy

digest I716101 low copy guy (EcoRI, BamHI), 3150+910, largest
digest I716008 ptet/rbs/wbbL/rbs/neuS (EcoRI, BamHI, AlwNI) 2146+1510+553, largest
Ligate them prod I716012 ptet/rbs/wbbL/rbs/neuS in lo copy